Karlovsky Lab  


of Molecular Phytopathology and Mycotoxin Research Group

Our bioassays: a synopsis

We study secondary metabolites of fungi and plant, focusing on their biological functions. Examination of biological effects of these metabolites in pure form, together with the knowledge of conditions stimulating or suppressing their synthesis, provide important information to develop and test our hypotheses.

In bacteria, fungi and nematodes we determine mortality (LD50). In plants we determine germination rate and IC50 for root length (Arabidopsis) and leave area (Lemna). In animals we investigate the effect of metabolites on fitness (length growth, weight gain).

Brine shrimps

Disassembled ion trap

Artemia salina hatching from eggs in artificial sea water.

Artemia salina

Dose-response curve of Artemia salina treated with a purified plant metabolite from Mohammad Alhussein.


Caenorhabditis elegans

We use three nematode species. Here Caenorhabditis elegans is shown, with large females and small males. The system was established by Zana Jamal Kareem.

Lemna minor bioassay

Lemna minor bioassay

Lemna minor bioassay

Duckweed is one of the oldest and still most frequently used test organism for phytotoxicity, particularly in the industry. The assay was established by Mohammad Alhussein.

Bioassay with isopods



We used several local and tropical isopod species. The assays were established by Yang Xu and used by Jessica Weller, Nilay Sal, Markus Arndt and others.